LOCUS pLenti-PGK-X-SV4 9313 bp DNA circular 19-FEB-2009 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|510418177| COMMENT VNTDBDATE|510418177| COMMENT LSOWNER| COMMENT VNTNAME|pLenti-PGK-X-SV40-Blast (524-1)| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers misc_feature 2216..2447 /vntifkey="21" /label=RSV\E/P promoter 2448..2628 /vntifkey="29" /label=5'UR misc_feature 3291..3531 /vntifkey="21" /label=RRE misc_feature 6326..6450 /vntifkey="21" /label=attR2 misc_feature 6476..7072 /vntifkey="21" /label=WPRE misc_feature 4746..4870 /vntifkey="21" /label=AttR1 misc_feature 4979..5638 /vntifkey="21" /label=CM(R) misc_feature 8054..8287 /vntifkey="21" /label=DU3RU5 exon 144..992 /vntifkey="61" /label=AMP exon 1138..1810 /vntifkey="61" /label=PUC promoter 4179..4694 /vntifkey="30" /label=hPGK promoter 7138..7447 /vntifkey="30" /label=SV40 loci 7569..7959 /vntifkey="18" /label=Blasticidine exon 4019..4136 /vntifkey="61" /label=cppt BASE COUNT 2387 a 2205 c 2375 g 2346 t ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga 4681 cctctctccc cagggggatc accggtcgtc gactagtcca gtgtggtgga attctgcaga 4741 tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata 4801 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc 4861 agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta 4921 taatgtgtgg attttgagtt aggatccggc gagattttca ggagctaagg aagctaaaat 4981 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca 5041 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat 5101 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca 5161 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga 5221 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac 5281 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc 5341 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa 5401 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc 5461 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga 5521 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt 5581 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaag 5641 atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg 5701 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg 5761 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg 5821 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg 5881 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa 5941 tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc 6001 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg 6061 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc 6121 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat 6181 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa 6241 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccgtt 6301 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta 6361 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt 6421 ttctcgttca gctttcttgt acaaagtggt tgatatccag cacagtggcg gccgctcgac 6481 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6541 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6601 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6661 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6721 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6781 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6841 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 6901 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6961 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 7021 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattctg 7081 cagatatccg gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt 7141 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag 7201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 7261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 7321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 7381 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 7441 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac 7501 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga 7561 actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg 7621 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta 7681 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag 7741 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg 7801 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc 7861 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag 7921 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca caattcgagc 7981 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 8041 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct 8101 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctgcctaggg 8161 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 8221 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 8281 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga 8341 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat 8401 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 8461 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca 8521 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 8581 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 8641 tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 8701 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 8761 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 8821 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag 8881 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 8941 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 9001 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 9061 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 9121 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 9181 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 9241 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 9301 gtttacaatt tcc //